Figure 2

Perfusion increased ECM deposition, in vitro pre-vascularization and pro-angiogenic factor release. Analyses performed on constructs cultured 5 days either in perfusion or static condition. (A) Representative H&E images. (B) Cell density quantification. (C) Representative Picro-Sirius Red images. *Identifies collagen scaffold. (D) Representative immunofluorescence images for cell proliferative marker Ki-67 (red). Nuclei were stained with DAPI (blue). (E) Quantification of the proliferating cells stained with Ki-67 normalized over the total amount of DAPI. (F) Representative immunofluorescence images for endothelial marker CD31 (red). Nuclei were stained with DAPI (blue). (G) Percentage of endothelial cell structures. *p < 0.05, ***p < 0.001. n.d. = not detectable. (n donor = 3). (H) Human angiogenesis proteome profiler array showing the release of angiogenic factors. Data are presented as mean pixel density normalized on DNA. § = *** p < 0.001; # = ** p < 0.01; ϵ = * p < 0.05. Scale bar: 50 μm. (n donor = 2).