Figure 3
Perfusion enriched the SVF for the vascular cell component. All population presented are considered CD45− (A–C). (A) In vitro cell phenotype characterization by 6-channel-flow-cytofluorimetric analysis of freshly isolated (Fresh) or SVF cells following static or perfusion culture. Quantification presented as percentage over the living cells (DAPI+) of different SVF subpopulations: pericyte (CD34− CD146+), MSC (CD73+ CD90+), EC (CD31+ CD34+) and mature EC (CD31+ VEGFR2+) (n donor = 6). (B) Graph representing the ratio of pericytes and EC - progenitor and mature - over the fresh SVF (red line indicates ratio = 1). (C) Scatter plot showing the distribution of the 6 donors after static or perfusion culture (red lines indicate the mean). *p < 0.05, **p < 0.01, ***p < 0.001. (D) Representative immunofluorescence images for pERK(1/2) (red). Nuclei were stained with DAPI (blue). Scale bar = 20 μm. (E) Quantification of the pERK(1/2)+ cells normalized over the total amount of DAPI. *p < 0.05, **p < 0.01, ***p < 0.001 (n donor = 2).
