Figure 4 | Scientific Reports

Figure 4

From: Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells

Figure 4

Perfusion accelerated in vivo vascularization of engineered tissues. (A) Representative immunofluorescence images stained for endothelial cells (CD31; green). Dashed lines outline the border between the patch and rat tissue (identified by the *). White arrows indicate branching points in perfusion condition at 28 days. Scale bar = 50 μm. (B) VLD quantification normalized over the analysed area. (C) Representative immunofluorescence images stained for EC (Ve-Cad; red) and intravascular marker (lectin; green) injected prior to sacrifice at 28 days. Nuclei were stained with DAPI (blue). Scale bar = 20 μm. (D) Percentage of the total vessels that resulted perfused by blood (lectin+) at the moment of sacrifice. *p < 0.05, ***p < 0.001. (n donor = 3).

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