Figure 6
Pericytes modulated the factor release and increased host response. (A) Human angiogenesis proteome profiler array between perfusion and perfusion−146+ supernatants. Data are presented as mean pixel density normalized on DNA. § = *** p < 0.001; # = ** p < 0.01; ϵ = * p < 0.05 (n donor = 3). (B) Low magnification pictures for three different conditions: perfusion, perfusionmocked (same technical procedure but no primary antibody), and perfusion−146+. 3 days in vivo time point. Scale bar = 1 cm. Representative images stained for Masson’s trichrome (C) and immunofluorescence images stained for hematopoietic cells (CD45; red) and macrophages (CD68; green) (D) and for hematopoietic cells (CD45, red) and human cells (HuNu, green) (E) of constructs generated in perfusion and perfusion−146+ conditions. Nuclei were stained with DAPI (blue). 3 days in vivo time point. Scale bar = 50 μm. White arrows indicate differentiated cells along a macrophage-lineage. Inset images in C and D bottom panels represent high magnification views with a scale bar = 12.5 μm.
