Table 1 Oligonucleotide primers used for PCR analysis.

From: Pharmacological inhibition of myostatin improves skeletal muscle mass and function in a mouse model of stroke

Gene

Primer sequence 5′-3′

GenBank® accession no.

β-actin

Fwd: AGCAAGCAGGAGTACGATGAG

NM_007393.3

Rev: AACGCAGCTCAGTAACAGTC

Atg5

Fwd: TGAAAGAGTGTGTCCTCCTC

NM_053069.5

Rev: GCCTCCACTGAACTTGACTG

Bnip3

Fwd: AGA TTG GAT ATG GGA TTG GTC AAG

NM_009760.4

Rev: CCC TTT CTT CAT AAC GCT TGT G

Cathepsin B

Fwd: GAA GAA GCT GTG TGG CAC TG

NM_007798.3

Rev: GTT CGG TCA GAA ATG GCT TC

Cathepsin L

Fwd: GTG GAC TGT TCT CAC GCT CAA G

NM_009984.3

Rev: TCC GTC CTT CGC TTC ATA GG

LC3b

Fwd: CACTGCTCTGTCTTGTGTAGGTTG

NM_026160.4

Rev: TCGTTGTGCCTTTATTAGTGCATC

MAFbx/atrogin-1 (Fbxo32)

Fwd: GTTTTCAGCAGGCCAAGAAG

NM_026346.3

Rev: TTGCCAGAGAACACGCTATG

MHC I

Fwd: AGTCCCAGGTCAACAAGCTG

NM_080728.2

Rev: TTCCACCTAAAGGGCTGTTG

MHC IIa

Fwd: AGTCCCAGGTCAACAAGCTG

NM_001039545.2

Rev: GCATGACCAAAGGTTTCACA

MHC IIb

Fwd: AGTCCCAGGTCAACAAGCTG

NM_010855.3

Rev: TTTCTCCTGTCACCTCTCAACA

MuRF1 (Trim63)

Fwd: ACCTGCTGGTGGAAAACATC

NM_001039048.2

Rev: AGGAGCAAGTAGGCACCTCA

Musa1

Fwd: TCGTGGAATGGTAATCTTGC

NM_001168297.1

Rev: CCTCCCGTTTCTCTATCACG

Ppia

Fwd: AGCATACAGGTCCTGGCATC

NM_008907.1

Rev: TTCACCTTCCCAAAGACCAC

Ulk1

Fwd: TTCCTGTCAGTCTGGCTCCT

NM_009469.3

Rev: TGAACAGAGCCGTGACAAAG

  1. Atg5: autophagy related 5; Bnip3: BCL2 interacting protein3; Fbxo30/Musa1: F-box protein 30/muscle ubiquitin ligase of the SCF complex in atrophy-1; MAFbx/atrogin-1: F-box protein 32; Map1lc3b/LC3b, microtubule-associated protein 1 light chain 3 beta. MHC I: type I myosin heavy chain; MHC IIa: type IIa myosin heavy chain; MHC IIb: type IIb myosin heavy chain; MuRF1 (Trim63): tripartite motif-containing 63; Ulk1: unc-51 like kinase 1. Ppia: peptidylprolyl isomerase A.
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