Figure 1 | Scientific Reports

Figure 1

From: Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues

Figure 1

Microphotographs of representative examples of validation from IHC (left panels), uniplex IF tumor-associated immune cell expression (middle panels), and details of uniplex IF in tonsil tissue (right panels). Immune panel 1: AE1/AE3 (cytokeratin-positive), PD-L1–positive, helper T cell (CD4-positive), cytotoxic T cell (CD8-positive), T-cell lymphocyte (CD3-positive), and macrophages (CD68-positive). ×200 magnification and high-power magnification of the positive cells.

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