Figure 2

Microphotographs of representative examples of validation from uniplex IHC (left panels), uniplex IF tumor-associated immune cell expression (middle panels), and details of uniplex IF in tonsil tissue (right panels). Immune panel 2: AE1/AE3 (cytokeratin-positive), PD-1–positive, granzyme B–positive, natural killer cell (CD57-positive), memory T cell (CD45RO-positive), and regulatory T cell (FOXP3-positive). ×200 magnification and high-power magnification of the positive cells.