Table 1 Densities of immune markers (in mm2) in the various staining batches.
Marker | Median density ( ± SD) of NSCLC specimens | |||
|---|---|---|---|---|
mIF Batch 1 | mIF Batch 2 | mIF Batch 3 | IHC | |
Panel 1 | ||||
AE1/AE3 | 3669.06 ± 776.610 | 3580.27 ± 1178.88 | 4502.54 ± 1383.93 | 3811.73 ± 846.840 |
PD-L1 | 3614.95 ± 1986.31 | 3310.91 ± 2495.13 | 3531.54 ± 2709.34 | 0540.51 ± 2127.47 |
CD4 | 0891.78 ± 1883.41 | 976.91 ± 975.64 | 2218.24 ± 1953.25 | 633.89 ± 605.81 |
CD8 | 539.61 ± 1403.7 | 107.92 ± 243.08 | 447.23 ± 339.02 | 0609.88 ± 1091.16 |
CD3 | 0891.78 ± 1883.41 | 976.91 ± 975.64 | 2218.24 ± 1953.25 | 1029.90 ± 1787.23 |
CD68 | 0899.55 ± 1374.03 | 1176.08 ± 836.590 | 1095.07 ± 683.480 | 457.70 ± 283.38 |
Panel 2 | ||||
AE1/AE3 | 3438.34 ± 1032.16 | 3496.26 ± 695.430 | 3769.51 ± 776.440 | 3811.73 ± 846.840 |
PD-1 | 01235.2 ± 1250.32 | 1239.46 ± 1065.86 | 1288.12 ± 766.640 | 170.10 ± 307.71 |
Granzyme B | 55.90 ± 91.55 | 124.96 ± 80.210 | 063.68 ± 118.02 | 398.51 ± 664.76 |
CD57 | 1025.41 ± 706.910 | 637.67 ± 445.85 | 1054.11 ± 798.790 | 22.12 ± 48.05 |
CD45RO | 1232.29 ± 1064.19 | 811.96 ± 513.33 | 1505.23 ± 1328.76 | 0797.09 ± 1096.41 |
FOXP3 | 0.00 ± 3.32 | 050.22 ± 118.49 | 1.49 ± 5.58 | 186.85 ± 159.02 |
