Figure 9

Flow cytometry analysis of ROS alteration in Zebrafish embryos exposed to different concentration of ZnO nanoparticles at 72hpf. Embryos were stained with ROS marker 2,7-dichlorodihydrofluorescein diacetate (H2DCFDA). (A) Histogram presentation of gated cells (B) Mean fluorescence intensity(MFI). All the measurements were taken in triplicate and the values were presented as mean ± SD of three independent experiments. *P < 0.05, •P < 0.05 denotes significant change from Untreated and Bulk ZnO treated embryos respectively. Number of *,• presents the degree of significance.