Figure 3 | Scientific Reports

Figure 3

From: microRNA-181b is increased in cystic fibrosis cells and impairs lipoxin A4 receptor-dependent mechanisms of inflammation resolution and antimicrobial defense

Figure 3

miR-181b and ALX/FPR2 expression in normal and CF-MΦs. MΦs and CF- MΦs were obtained by exposing peripheral blood monocytes from 3 healthy volunteers (HS) and 3 CF patients to GM-CSF for seven days. (A) miR-181b levels were determined by real-time PCR. Bars represent mean ± SEM. **p = 0.009. (B) ALX/FPR2 expression in MΦs and CF-MΦs were evaluated by flow cytometry. Data are mean ± SEM. **p = 0.0019. The inset shows a representative histogram. (C) Normal MΦs were exposed to vehicle (CTRL) or CFTRinh-172 (10 µM) for 30 min. miR-181b levels were determined by real-time PCR. Results are mean ± SEM from n = 3 with duplicates. **p = 0.001. (D) Total ALX/FPR2 levels in normal MΦs exposed to vehicle (CTRL) or CFTRinh-172 (10 µM) levels in were quantitated by flow cytometry. Bars represent mean ± SEM from n = 3. **p = 0.0019. The inset shows a representative histogram.

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