Figure 1
From: Structure determination and activity manipulation of the turfgrass ABA receptor FePYR1
Identification of putative ABA receptors in turfgrass. (A) SDS-PAGE gel showing purified H6-SUMO tagged candidate ABA receptor proteins, H6-SUMO-PYR1 and biotin-MBP-HAB1. (B) Alpha-Screen assay for the interactions between ABA receptors and AtHAB1 in the presence of 100 μM ( + )-ABA (n = 3, error bars are means ± SD). DMSO and H2O were used as negative controls. (C) Sequence alignment between the three candidate turfgrass ABA receptors and the subfamily III members of Arabidopsis ABA receptors. The secondary structure elements of FePYR1 were highlighted by wave lines above the alignment. Conserved residues forming the gate and latch are colored in red. (D) ABA dose-dependent interactions between AtHAB1 and PYLs as determined by Alpha-Screen assays. The table at the lower panel shows the EC50 values for the interactions (n = 3, error bars are means ± SD).
