Figure 1

Principles of tAS-PCR, cAS-PCR, and rcAS-PCR. The principles of tAS-PCR, cAS-PCR, and rcAS-PCR are shown in panels a, b, and c, respectively. In the tAS-PCR system, only primers and probes targeting MT-alleles (e.g., V600E) were used (i.e., ASP, LSP1, and LST1). In the cAS-PCR, in addition to the oligonucleotides in the tAS-PCR system, artificially introduced external templates (i.e., CEAC plasmids) and corresponding probes (i.e., LST2) were used. In the rcAS-PCR, in addition to the oligonucleotides and artificial templates used in cAS-PCR system, additional primers and probes targeting RIPC (e.g., the leptin gene) were used (i.e., LSP2, LSP3, and LST3). In the current study, the corresponding oligonucleotides used were: ASP is HQ-663, -666, or -668; LSP1 is HQ-675; LSP2 is HQ-329; LSP3 is HQ-330; LST1 is HQ-467; LST2 is HQ-648; LST3 is HQ-1294 (Table 1).