Figure 4
The suppressive effects of cAS-PCR for BRAF V600E WT-alleles. Experiments were performed with a reaction mixture containing the indicated concentrations of WT-gDNA and serial concentrations of CEAC plasmids. The C q values of WT-gDNA and CEAC plasmids are indicated using red and blue lines, respectively. For the reaction mixture that did not show positive amplification signals after 50 thermal cycles, the C q values were recorded as 50 to simplify the analysis. In the reaction without CEAC, the mean C q values of WT-gDNA at final concentrations of 50 ng, 5 ng, and 500 pg were 34.30 ± 0.27, 37.62 ± 1.46, and 40.34 ± 1.43, respectively. In the cAS-PCR reactions containing 1,000 copies of the CEAC plasmid, the amplifications were eliminated after 50 cycles with any concentration of WT-gDNA.
