Figure 6 | Scientific Reports

Figure 6

From: Improved detection of BRAF V600E using allele-specific PCR coupled with external and internal controllers

Figure 6

Standard curves showing the selectivity of cAS-PCR and rcAS-PCR. Panels a and b show the standard curves generated by plotting the average C q values of MT-alleles from real-time cAS-PCR and rcAS-PCR, respectively, against the log percentages of V600E MT-alleles (0.1%, 0.5%, 1%, 10%, 25%, 50%, and 100%) present in 100 ng of WT- and MT-gDNA. The mean C q values were obtained from Figures S1, respectively. These were determined automatically by using CFX Manager™ Software v3.1. The mean C q values of CEAC plasmids were 31.21 ± 0.41 and 31.11 ± 2.62 in panels a and b, respectively; the mean Cq value of RIPC (i.e., leptin) was 27.58 ± 0.38 (panel b).

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