Figure 6

TMRE uptake decreases and 2-NBDG uptake increases in dorsal window chambers under hypoxic gas breathing (10% oxygen). Normal dorsal window chambers were imaged with TMRE (first) and 2-NBDG (second) with a 10–15 min delay between injections. TMRE uptake and 2-NBDG uptake were captured under either normoxia or hypoxia. (a) Representative images for each test group. (b) Mean uptake kinetics of TMRE. (c) Mean uptake kinetics of 2-NBDG. (d) Pixel distribution curves show the mean distribution of pixels from TMRE images taken at 45 minutes (TMRE₄₅) for each group. (e) Pixel distribution curves show the mean distribution of pixels of delivery-corrected 2-NBDG images taken at 60 minutes (2-NBDG₆₀/R_D) for each group. (f) Mean intensity from TMRE₄₅ images. (g) Mean intensity from 2-NBDG₆₀/R_D images. NS = not significant. N = 4–5 mice/group. Comparison of mean kinetic curves across animal groups was performed with a two-way Analysis of Variance (ANOVA) test using the MATLAB (Mathworks, USA) statistics toolbox. Comparison of mean pixel distribution curves across animal groups was performed with a Kolmogorov-Smirnov (KS) test using the MATLAB (Mathworks, USA). Comparison of the mean intensity of TMRE₄₅ or 2-NBDG₆₀/R_D across animal groups was performed with two sample t-tests using the MATLAB (Mathworks, USA) statistics toolbox.