Figure 5

Adhesion test and live/dead assay of NIH-3T3 cells on the surface modified PTFE substrates. (a) Optical and fluorescence microscopy images representing the adhesivity and live/dead assay of the NIH-3T3 cell line stained with DAPI(blue), FDA(green), and PI(red) reagents (scale bar: 100 μm). (b) Relative viability of NIH-3T3 cell on the PTFE surfaces coated with DOPA-C7 assemblies. Relative viability of TCP: 1.00 ± 0.03; uncoated PTFE: 1.04 ± 0.18; DOPA-C7 coated PTFE: 2.46 ± 0.35; tannic acid coated PTFE: 1.34 ± 0.14; hydrocaffeic acid coated PTFE: 0.51 ± 0.33; and pyrocatechol coated PTFE: 1.57 ± 0.25 (p < 0.05).