Figure 1
From: MIF Inhibitor ISO-1 Protects Photoreceptors and Reduces Gliosis in Experimental Retinal Detachment
Proteomic analysis of murine RD. (a) Heatmap of proteins with FDR < 0.05 identified on iTRAQ proteomic screen. Proteins extracted from individual murine retina plus vitreous at week 2 (n = 3) or week 4 (n = 3) post RD creation were labeled with iTRAQ probes in an 8-plex assay. Control murine retinas (n = 2) were sham-treated (label #119) or untreated (#121). Red represents strong protein expression and blue represents lack of expression. Validation of up-regulated proteins in homogenized retina plus vitreous was performed by ELISA at four timepoints post RD for MIF with n = 15 individual mice/group. MIF expression was significantly elevated in RD eyes at week 1 (b, *p = 0.0430).
