Figure 6
From: MIF Inhibitor ISO-1 Protects Photoreceptors and Reduces Gliosis in Experimental Retinal Detachment
Retinal pERK is up-regulated in ISO-1 and MIFKO RDs. Immunofluorescence staining shows pERK (green) accumulation in Müller glia after RD (b,c,e,f) compared with attached controls (a,d) in vehicle or ISO-1 treated animals at day 3 or day 14 (n = 6/group). Treatment with ISO-1 displayed significant elevation in pERK expression in day 14 eyes (k, *p = 0.0356) while pERK expression in day 3 detached retinas was not significantly elevated (k, p = 0.1948). Detached retinas in day 14 BALB/c wild type controls (h) and MIFKO mice (j) displayed increased pERK accumulation (red) compared to attached retina in controls (g,i). Detached retina in MIFKO mice had significantly elevated pERK accumulation compared with background mice (l, n = 5/group, *p = 0.0246). Scale bar denotes 50 microns.
