Figure 4 | Scientific Reports

Figure 4

From: Alpha-1 antitrypsin augmentation therapy decreases miR-199a-5p, miR-598 and miR-320a expression in monocytes via inhibition of NFκB

Figure 4

NFκB modulation regulates (a), miR-598, (b), miR-199a-5p, and (c), miR-320a expression. Relative expression of miRNAs normalized to U6 snRNA was determined using Taqman assays in asymptomatic ZZ monocytes (1 × 105 cells, n = 3, separate cultures in triplicate) treated with or without 20uM BAY-11 7082 or 500U/ml TNF-α or both for 4 hours. (d), Relative expression of miR-598, miR-199a-5p, miR-320a and miR-30a was determined using the TaqMan miRNA assays in asymptomatic MM monocytes (1 × 105 cells) and MM monocytes treated with 50 nM of thapsigargin for 1 hour. Assays were performed n = 3, in triplicate and data are represented as mean ± SEM (a and b ***p < 0.0001, c ***p = 0.0007 measured by ANOVA with Bonferroni’s correction for multiple group comparison. d *p ≤ 0.0167 measured by t-test with Bonferroni correction).

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