Figure 3
Membrane targeting, amplitudes and reversal potentials of eACRs. (A) HEK cells expressing the established eACRs iC++, iChloC and their C128A variants or the newly generated eACRs Phobos and Aurora and their C128A variants fused to the mCherry were co-labelled with the membrane dye Vybrant®-DiO (middle row). Upper row: confocal images of mCherry, lower row: merged images of eACR-mCherry and labelled cell membrane (equatorial z-slices). Intensity and/or contrast for DiO were adjusted due to different staining efficiency. (B) Relative membrane targeting of eACRs (n = 14 Phobos, 12 PhobosCA, 13 iC++, 13 iC++CA, 11 Aurora, 12 AuroraCA, 18 iChloC, 21 iChloCCA). (C) Absolute stationary photocurrents of eACRs at indicated conditions (n = 6 Phobos, 5 PhobosCA, 6 iC++, 6 iC++CA, 5 Aurora, 7 AuroraCA, 9 iChloC, 7 iChloCCA). (D) Reversal potentials (Erev) for all eACRs at high-extracellular (150 mM) and low-intracellular (10 mM) chloride concentrations (n = 5 Phobos, 5 PhobosCA, 6 iC++, 5 iC++CA, 8 Aurora, 5 AuroraCA, 7 iChloC, 6 iChloCCA). Bar plots show mean ± SEM. Single measurement data points are shown as dots.
