Figure 6
eACRs in Drosophila larval nociception and locomotion. (A) Mechano-nociceptive responses (rolling) of 3rd instar larvae after 50 mN stimulation with a von Frey filament, with and without light activation of C4da neurons (ppk-Gal4) expressing Halorhodopsin (2x eNpHR), iChloC or Aurora. No light stimulus (gray), 460–495 nm (blue) and 545–580 nm (yellow) conditions are shown as indicated by color (n as indicated, ***p < 0.001, chi2 test). (B) Representative traces of freely locomoting larvae expressing Aurora in motor neurons (ok371-Gal4). Arrows indicate onset (green, 525 nm, 21 µW/mm2) and offset (gray) of light activation. Relative velocity is color intensity coded in red. (C) Average larval velocity over time is plotted for Phobos (top) or Aurora (bottom) expressing animals and respective wild-type controls (ok371-Gal4) with activation using a 460 nm or 525 nm light pulse for 15 s, respectively (n = 49 animals for Phobos, n = 52 animals for Aurora, mean ± SEM). (D and E) Averaged velocity before (5–10 s), during (20–25 s) and after (35–40 s) light induced activation of (D) Phobos or (E) Aurora (n = 49 animals for Phobos, n = 52 animals for Aurora, mean ± SEM). (F) Relative velocity reduction during light for Phobos, Aurora and control animals using 460 nm or 525 nm light, respectively (mean ± SEM, ***p < 0.001, ****p < 0.0001, non-parametric Kruskal-Wallis test followed by Dunn’s multiple comparisons test).
