Figure 1
From: Asymmetric Sensory-Motor Regeneration of Transected Peripheral Nerves Using Molecular Guidance Cues
Experimental design. (A) Schematic of Y-conduit containing microparticles with a neurotrophic factor in one compartment and BSA neutral control in the other. The transected mixed sciatic nerve is placed in the common arm of the Y-conduit and distal ends are capped with 1.5% agarose. (B) Timeline of the experimental procedures following implantation. (C) PTN cumulative release curve over 30 day period and scanning electron microscope of PTN-MP with size ranging from 0.3–1.5 µm (inset; Scale bar = 3 µm). (D) Compared to control and BSA MP, the average axonal length in neonatal DRGs for PTN and PTN MP was significantly higher (N = 3/group; One-way ANOVA; F (3, 263) = 33.52; R2 = 0.2766; ***indicates P ≤ 0.001). Data represented at mean ± SEM. (E,F) Representative images of DRGs exposed to PTN and PTN-MP.
