Figure 2

Stopped-flow measurements with S448C Atg18 and S448C FTTG Atg18. Time courses of Texas Red (A and B) or Rhodamine B (E and F) fluorescence emission measurements at different accessible lipid concentrations are shown for S448C Atg18 (A and E) and S448C FTTG Atg18 (B and F). A scale-up of the the traces in Fig. 2B and fitting results are shown in Supplementary Figure 11 and Supplementary Tables 3–7, respectively. (C and G) Graphs showing the dependency of k _obs1 from the accessible lipid concentration. The y-intercept provides the dissociation rate constant k _off, and the slope yields the association rate constant k _on. Bars represent the standard errors of three to five technical repeats. The solid line shows a weighted linear fit (equation 2 in Methods). Amplitudes of the k _obs1 used for data analysis are shown in Supplementary Figure 5. (D and H) Increases of Texas Red (D) or Rhodamine B (H) fluorescence emission are shown at different accessible lipid concentrations for S448C Atg18 and S448C FTTG Atg18.