Figure 2
From: Conformational stabilization as a strategy to prevent nucleophosmin mislocalization in leukemia
Hit compounds C1, C2 and C3 bind NPM, stabilizing the C-terminal domain. DSF profiles of NPM C-terminal domain in the absence (black line) and presence (blue line) of hit compounds C1, C2 and C3, and chemical formulae of the compounds (a). Isotherms for compound hits (C1, C2 and C3) binding to NPM analyzed by surface plasmon resonance (b). Experiments were performed in triplicate with each inset depicting a representative original sensorgram at compound concentrations 0, 7.81, 15.6, 31.2, 62.5, 125, 250 and 500 µM (corresponding increase in color intensity), all in 5% DMSO. S 0.5 values of 68 ± 13 µM for C1, 105 ± 17 µM for C2 and 102 ± 23 µM for C3 were obtained from fittings to a 1:1 binding model.
