Figure 3
From: Conformational stabilization as a strategy to prevent nucleophosmin mislocalization in leukemia
Effects of C1 on circular dichroism, fluorescence emission and thermal behavior of NPM mutant E C-terminal domain. A synthetic peptide corresponding to the C-terminal domain of mutant E was diluted in 50 mM phosphate buffer (pH 7.5) to a final concentration of 50 μM. Circular dichroism spectra (a), Trp fluorescence emission spectra (b) and thermal scans (c) were recorded in the absence (solid line) and presence (dashed line) of 150 µM C1. For CD experiments (a), C1 was prepared in ethanol, whereas for fluorescence spectroscopy (b) and (c), C1 was prepared in DMSO. In all cases, the final concentration of solvent was 1.5% (v/v).
