Figure 2
From: Krüpple-like-factor 4 Attenuates Lung Fibrosis via Inhibiting Epithelial-mesenchymal Transition
Overexpression of KLF4 inhibited bleomycin-induced pulmonary fibrosis in vivo. Overexpression of KLF4 in the transgenic mice were confirmed with PCR (A) and western-blotting (B). Mice were intratracheally intubated and injected with bleomycin to induce pulmonary fibrosis Saline was used as a control, in overexpression of KLF4 mice group and wild-type group. H&E staining (C) and Masson staining (D) were used to detect collagen depositions. Ashcroft scores showed the degree of fibrosis (E). The lung tissues of mice were harvested and assayed for hydroxyproline analysis (F). The expression of E-cadherin and fibronectin was evaluated by western blotting (G) in KLF4 overexpressing group and wild-type group. Representative images were from five mice per group. Scale bar = 200 μm. The values were expressed as mean ± SD from N = 5 in each group. *P < 0.05 and **P < 0.01 by t-test.
