Figure 5
From: Krüpple-like-factor 4 Attenuates Lung Fibrosis via Inhibiting Epithelial-mesenchymal Transition
Knockdown of KLF4 promoted TGF-β1-induced EMT in alveolar epithelial cells. AECs were transfected with KLF4 siRNAs or control siRNA (100 nM) for 48 h. KLF4 expression level was assessed with qRT-PCR (A). AECs transfected with siRNA or control siRNA were stimulated with TGF-β1 for 48 hours. The TGF-β1 induced morphological changes were shown (B). Total proteins were immunoblotted with antibodies against E-cadherin and fibronectin. β-actin was used as loading control (C). Immunocytochemistry staining of E-cadherin and fibronectin in KLF4 knock down and control AECs (D). (E) RNA interference of KLF4 F-actin staining (Green) was shown. Nuclei were counterstained with DAPI. ((A) Scale bar = 50 μm. (B). Scale bar = 20 μm). Data shown are representative of three independent experiments.
