Figure 6
From: Krüpple-like-factor 4 Attenuates Lung Fibrosis via Inhibiting Epithelial-mesenchymal Transition
KLF4 inhibited TGF-β1-induced phosphorylation of Smad2/3 and Dvl. AECs were co-infected with AdKLF4 and AdtTA in the medium with or without tetracycline and then stimulated with or without TGF-β1 (5 ng/mL) for the indicated time (A,E). AECs were transfected with KLF4 siRNAs or control siRNA (100 nM) and then were stimulated with TGF-β1 (5 ng/mL) for indicated time (B,F). Total protein lysate was immunoblotted with antibodies against Smad2/3, their phosphorylated forms, DVL-2 and β-actin. Densitometry analysis of figure A and B was performed with imageJ (C,D). Similar results were obtained in three independent experiments.
