Figure 5
Morphology of testis and epididymis in targeted deletion mutants. (a) Testes from 8-week-old control and targeted mutant mice (Exosc10 cKO(Stra8) and Exosc10 cKO(Vasa)) are shown. Scale bar: 2 mm. (b) A color-coded graph plots the weight of testis over body-weight (y-axis) for controls (C in blue) and Stra8-cre mutants (Exosc10 cKO(Stra8) in purple) at the ages indicated at the bottom (x-axis). (P14: control, n = 6; mutant, n = 4; p = 0.171/2–3 months: control, n = 7; mutant, n = 11/8–12 months: control, n = 10; mutant, n = 12; ***p < 0.001, non parametric Mann-Whitney test). The data are presented as median values ± SD. (c) Epididymides from 8-week-old control and mutant mice are shown. Scale bar: 2 mm. (d) A color-coded graph plots the weight of epididymides over the body weight. (2–3 months: control, n = 6; mutant, n = 8; *p < 0.05/8–12 months: control, n = 7; mutant, n = 6; p = 0.0734, non parametric Mann-Whitney test). The data are presented as median values ± SD. (e) The number of spermatozoa over the weight of epididymis is plotted. (control, n = 3; mutant, n = 4, *p < 0.05, non parametric one-tailed Mann-Whitney test). The data are presented as median values ± SD. (f) Hematoxylin/eosin staining of paraffin-embedded epidydimis tails from control, Exosc10 cKO(Stra8) and Exosc10 cKO(Vasa). Black arrowheads point to spermatozoa. Scale bar: 20 μm.
