Figure 5

The effects of DHI on nuclear translocation of c-Jun, p38, NF-κB p65 induced by different stimulants. EA.hy926 cells were pre-incubated with DHI for 1 h before adding 10 μg/mL LPS or 100 μg/mL ox-LDL, and cultured for 30 min. (A) Representative photo-images and (B) summary bar graph of DHI at different dilution ratio (1/3200, 1/1600 and 1/800) on 10 μg/mL LPS induced p-c-Jun, p-p38 or p-NF-κB p65 nuclear translocation. (C) Representative photo-images and (D) summary bar graph of different dilution ratio of DHI on 100 μg/mL ox-LDL induced p-c-Jun, p-p38, or p-NF-κB p65 nuclear translocation. (E) CHC (10–500 μg/mL) on p-c-Jun, p-p38 or p-NF-κB p65 nuclear translocation. Nucleus were stained by Hoechst (blue) and the transcriptional factors were stained by immunolabeled antibodies for p-c-Jun (green), p-p38 (yellow), or p-NF-κB p65 (red). Cells were imaged with the HCA reader using a 20× objective lens with each column reflecting images collected from the respective fluorescent channels using the same optical field. Data are presented as mean ± SD (n = 3). **P < 0.01 versus control; ^#P < 0.05 versus LPS or ox-LDL group; ^##P < 0.01 versus LPS or ox-LDL group.