Figure 8

Anti-inflammatory screening of individual DH ingredients. (A) Sub-network of 14 ingredients predicted targeting NF-κB p65by network analysis. (B) EA.hy926 cells were cultured with each of the 14 DH ingredients (1 μM) for 1 h before adding 10 μg/mL LPS for 30 min. NF-κB p65 nuclear translocation assay was performed and quantification of the data was shown in bar graph. (C) Representative fluorescence microscopic images of apigenin effect on LPS-induced NF-κB p65 nuclear translocation. Nucleus were stained by Hoechst (blue) and the NF-κB p65 were stained by immunolabeled antibodies (green). Cells were imaged with the HCA reader using a 20× objective lens and then amplified to see the details, each column reflecting images collected from the respective fluorescent channels by the same optical field. Data are presented as mean ± SD (n = 3). **P < 0.01 versus control; ^#P < 0.05 versus LPS group; ^##P < 0.01 versus LPS group.