Figure 3 | Scientific Reports

Figure 3

From: Application of Lysine-specific Labeling to Detect Transient Interactions Present During Human Lysozyme Amyloid Fibril Formation

Figure 3

Intramolecular PRE effects induced by nitroxide-spin labels at three individual lysine residues (K33 in I59T, K13 or K97 in the I59T-K33R) at pH 5, 37 °C. Intensity ratios of the correlation peaks in the paramagnetic and diamagnetic samples were measured and used for mapping the structures. The intensity ratios that appear higher than 1.0, due to experimental error, are mapped as 1.0. Low and high PRE effects are shown in blue and red, respectively. Residues where PRE values could not be measured are shown in dark grey and the yellow arrows indicates the location of the nitroxide spin labels. The intramolecular PREs of natively folded I59T with the nitroxide label at K33 show an effectively identical PRE profile to the K33 spin labeled WT protein (see Supplementary Fig. S2). The PRE effects from spin labels located at K97 and K13 relate residues which are close to the spin label either in the amino acid sequence (residues 85–105 for K97; residues 5–30 for K13) or in spatial terms (residues 10–25 for K97; residues 125–130 for K13).

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