Figure 5
Temperature evolution of the intermolecular PREs for I59T lysozyme (pH 1.2, at 35, 40, 45 and 50 °C) with a K33 nitroxide spin label. HSQC spectra of the diamagnetic sample at each temperature are shown on the left. The spectra at 25 and 45 °C with assignments are shown in Supplementary Figs S5 and S6, respectively. 1HN-Γ2 values (defined as the difference between 1HN-R2, para and 1HN-R2, dia) were calculated from 1HN-R2 values measured using two relaxation delay times (7, 37 ms) at 700 MHz. Red shading indicates regions of the N-terminus and the small loop at the domain interface where noticeable PREs are observed. Grey squares and black circles represent 1HN-Γ2 values measured from native and non-native peaks, respectively.
