Figure 6
(a) Intermolecular PREs for I59T lysozyme (pH 1.2, 40 °C) with spin labels at three different locations: K33, K97 and K13. 1HN-Γ2 values were calculated from the 1HN-R2 values measured using two relaxation delay times (7, 37 ms) at 700 MHz and used for mapping the structures. Red shading indicates regions of the N-terminus and the small loop at the domain interface where noticeable PREs are observed as in Fig. 3. (b) 1HN-R2 of I59T with spin label at K33 at 40 °C and the structure colored by 1HN-R2 of the diamagnetic sample. Filled squares and open circles indicate 1HN-R2 of the paramagnetic and diamagnetic samples, respectively. Data points from diamagnetic samples are used for mapping the structure. Blue shading indicates regions of the N-terminus and the β-hairpin regions where low 1HN-R2 values are observed (<15 s−1).
