Figure 7

Schematic diagram of the proposed unfolded β-domain region involved in early intermolecular interactions of I59T lysozyme. The structural elements shown in blue represent the regions that are predominantly unstructured and flexible (¹H_N-R₂ < 10 s⁻¹) (Fig. 3(b)), whereas the regions experiencing high PREs (¹H_N-Γ₂ > 10 s⁻¹) are shown in red. Green circles mark the nitroxide spin labels that are located at the three lysine residues (K13, K33 and K97, Fig. 3(a)). The black lines indicate the four disulfide bonds. Dashed lines indicate the potential intermolecular contacts giving rise to significant ¹H_N-Γ₂ values.