Figure 5
Analysis of systemic toxicity by flow cytometry and histology. (a) Flow cytometry analysis of total cells harvested from the lungs of non treated (No T) or treated (TM CAR) mice. GFP and CD3 were used as markers of 8505 C tumor and T cells, respectively. Mice treated with TM CAR T cells were sacrificed at different time points to represent the association between tumor burden and TM CAR T cells. (b) Lung tissues harvested from untreated mice and mice treated with TM and F292A CAR T cells were processed for H&E, GFP and CD3 staining. The scale bar in each image is 2 mm. Tissue sacrifice time points are indicated on the left. For example, X20T10 represents 20 days post tumor xenograft and 10 days post T cell infusion. (c) Murine ICAM-1 expression in lung cells harvested from tumor-bearing mice after treatment with TM CAR T cells.
