Figure 3

Characterization of 1a, 1i, and 3a binding to PPARγ. (A) Concentration-response curves of Rosiglitazone, Pioglitazone, 1a, 1i, and 3a in HepG2 cells co-transfected with pGal5TKpGL3 reporter and pGal4-hPPARγ-LBD. (B) SPR sensorgrams achieved injecting different concentrations (from 0.5 to 10 µM) of compounds 1a, 1i, and 3a on immobilized PPARγ. The positive control Rosiglitazone was injected at concentrations from 0.1 to 100 nM.