Figure 3 | Scientific Reports

Figure 3

From: Functional coupling of human pancreatic islets and liver spheroids on-a-chip: Towards a novel human ex vivo type 2 diabetes model

Figure 3

Pancreatic islet microtissues stay functional in co-culture with liver spheroids over 15 days. (A) Insulin accumulated over 48-h periods measured in the media of co-cultures and islets alone. Data shown as mean + SD, n = 5. (B) GSIS of 11 single islet microtissues in low (2.8 mM, black dots) and high (16.8 mM, white dots) glucose. *p < 0.05 using ANOVA with Tukey’s multiple comparison post hoc test. (C) Protein expression of insulin (red) and glucagon (green) in islet microtissues at day 15 in co-culture. Nuclei stained with DAPI (blue). Scale bar: 50 µm.

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