Figure 2 | Scientific Reports

Figure 2

From: High throughput discovery of influenza virus neutralizing antibodies from phage-displayed synthetic antibody libraries

Figure 2

Results of biopanning of the synthetic antibody libraries against immobilized HA trimer of H1N1 CA/09. (a) 15 GH2 phage-displayed scFv libraries were selected for HA binding. The y-axis shows the ratio of the output/input titer of the phage library in each of the biopanning rounds (x-axis). (b) The polyclonal soluble scFvs secreted in the E. coli culture media of the output phage libraries were assayed for HA trimer binding with ELISA (y-axis) for the biopanning rounds (x-axis) with each of the 15 phage-displayed scFv libraries. (c)~(d) The descriptions are the same as in (a) and (b) respectively for the biopanning protocol of the Step 1b in Fig. 1. The experiments were repeated twice, as shown in the panels. Experimental details are described in Methods.

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