Table 1 Oligonucleotide primers and PCR conditions of rat in quantitative real-time PCR.

From: High-fat diet and chronic stress aggravate adrenal function abnormality induced by prenatal caffeine exposure in male offspring rats

Genes

Forward primer

Reverse primer

Product (bp)

Annealing

StAR

GGGAGATGCCTGAGCAAAGC

GCTGGCGAACTCTATCTGGGT

188

65 °C, 30 s

CYP11A1

GCTGCCTGGGATGTGATTTTC

GATGTTGGCCTGGATGTTCTTG

188

63 °C, 30 s

CYP21

AGGAGCTGAAGAGGCACAAG

GAGGTAGCTGCATTCGGTTC

188

63 °C, 30 s

CYP11B1

CCCCTTTGTGGATGTGGTAG

CACGCTCTCAGGTTTCAGGT

188

61 °C, 30 s

3β-HSD

TCTACTGCAGCACAGTTGAC

ATACCCTTATTTTTGAGGGC

271

58 °C, 30 s

IGF1

GACCAAGGGGCTTTTACTTCAAC

TTTGTAGGCTTCAGCGGAGCAC

148

60 °C, 30 s

IGF1R

GTCCTTCGGGATGGTCTA

TGGCCTTGGGATACTACAC

188

62 °C, 30 s

Akt1

ATGAGCGACGTGGCTATTGTGAAG

GAGGCCGTCAGCCACAGTCTGGATG

156

60 °C, 30 s

SF1

CCAGTACGGCAAGGAAGA

GAGGCTGAAGAGGATGAGGA

188

63 °C, 30 s

11β-HSD1

GAAGAAGCATGGAGGTCAAC

GCAATCAGAGGTTGGGTCAT

133

63 °C, 30 s

11β-HSD2

TGGCCAACTTGCCTAGAGAG

TTCAGGAATTGCCCATGC

76

63 °C, 30 s

MR

TGCATGATCTCGTGAGTGA

GAGGCCGTCAGCCACAGTCTGGATG

156

63 °C, 30 s

GR

CACCCATGACCCTGTCAGTC

AAAGCCTCCCTCTGCTAACC

156

63 °C, 30 s

GAPDH

GCAAGTTCAATGGCACAG

AAGTTCTTCCTGGCCGGTAT

140

63 °C, 30 s

  1. StAR: steroidogenic acute regulatory protein, CYP11A1: cytochrome P450 cholesterol side chain cleavage, 3β-HSD: 3β-hydroxysteroid dehydrogenase, CYP21: steroid 21-hydroxylase, CYP11B2: steroid 11β-hydroxylase, IGF-1: insulin-like growth factor-1, IGF-1R: IGF-1 receptor, AKT1: protein kinase B, SF1: steroidogenic factor 1, 11β-HSD1: 11β-hydroxysteroid dehydrogenase type 1, 11β-HSD2: 11β-hydroxysteroid dehydrogenase type 2, MR: mineralocorticoid receptor, GR: glucocorticoid receptor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
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