Figure 3

Hypothesis-driven canonical correspondence analysis (CCA) of fecal microbiota compositions in the experimental groups of subjects. CCA was performed based on abundance of classified OTUs with frequency >99.9% in patients with established SSc and gastrointestinal symptoms (SSc/GI+), patients with SSc without gastrointestinal symptoms (SSc/GI-), and healthy controls (HC), respectively. The plot shows that the disease status is a significant source of variability in bacterial communities, explaining 13.2% of the variance in fecal microbiota. The percentages on each axis indicate the variation in the samples. Individuals are labeled according to the three groups studied.