Figure 1
From: Female mice lacking Pald1 exhibit endothelial cell apoptosis and emphysema
Paladin has a broad expression in the postnatal lung. (a) Endothelial cells and pneumocytes type II were isolated from lungs of mT/mG mice expressing endothelial-specific Tie2-Cre (left) or pneumocyte type II-specific SPC-Cre (right). Q-PCR of sorted single cells indicates Pald1 mRNA expression in both endothelial cells and non-endothelial cells, including pneumocytes type II, both at 3 weeks and 3 months of age. Expression was normalized to 18 S RNA. (b) Pald1 LacZ reporter activity (blue) is detected in Pald1 LacZ/LacZ mice, 5 days, 4 weeks and 19 weeks after birth. LacZ is broadly expressed in the lung tissue, except for the bronchial epithelium, which shows no reporter activity (arrow). Scale bar = 200 µm.
