Figure 2
From: Female mice lacking Pald1 exhibit endothelial cell apoptosis and emphysema
Paladin is expressed both in the epithelial and mesenchymal compartment of the postnatal lung (4 weeks). (a–e) Combined X-gal (black) and immunofluorescence staining of Pald1 LacZ/LacZ mice show Pald1 LacZ expression in the vasculature, i.e. endothelial cells in capillaries (a) but to a lesser extent in endothelial cells of larger blood vessels (b) as indicated by Erg staining (endothelial cell nuclei, green). In large blood vessels LacZ expression can be detected in vascular smooth muscle cells (c, α-smooth muscle actin, red). Paladin LacZ reporter is also active in pneumocytes type II (d, SPC, green) and pneumocytes type I/II (e, cytokeratin, red). Scale bar = 20 µm.
