Figure 5

Puerarin downregulated NOX4 expression through activation of SIRT1 in murine podocytes exposed to high glucose. (A,B) Murine podocytes cultured in NG or HG were treated with different amounts of puerarin as indicated. The cells were lysed for western blot analysis for SIRT1, NOX4, acetyl-p65, total-p65, and β-actin. Representative blots of three independent experiments are shown in A, and quantification is shown in (B). (C,D) Podocytes transfected with SIRT1 overexpression vector or control vector were cultured in NG and HG for 24 hours. The cells were lysed for western blot analysis for SIRT1, NOX4, acetyl-p65, total-p65, and β-actin. Representative blots of three independent experiments are shown in (C), and quantification is shown in (D).