Figure 6
From: Puerarin attenuates diabetic kidney injury through the suppression of NOX4 expression in podocytes
Puerarin downregulated NOX4 expression through deacetylation of p65NF-κB in murine podocytes exposed to high glucose. (A,B) Podocytes transfected with either wildtype p65 (WT), p65 with mutation of K310 acetyl-residue (K310R) or control empty vector (EV) were treated with TNF-α for 24 hours. Cells were lysed for western blot analysis for p65, NOX4 and β-actin. Representative blots of three independent experiments are shown in A, and quantification is shown in (B). (C) Podocytes cultured in NG or HG were treated with 10 μM puerarin or 1 μM SIRT1 agonist SRT1720 for 24 hours. Superoxide production was then measured by the DCF fluorescence method. *p < 0.05 compared between groups n = 3. (D) Real-time PCR analysis of Sirt1 mRNA in podocytes treated with vehicle or puerarin in NG or HG conditions show increased Sirt1 expression by puerarin. *p < 0.05 compared between groups n = 3.
