Figure 4
From: Ly6Chi inflammatory monocytes promote susceptibility to Leishmania donovani infection
STAT1 plays a critical role in iMO accumulation in L. donovani infected tissues. (A) Flow cytometric analysis of Ly6Chi cells from the spleen of L. donovani infected WT and STAT1KO mice at 20 dpi. (B) Flow cytometric analysis of Ly6Chi cells from the liver of L. donovani infected WT and STAT1KO mice at 20 dpi. (C) Bone marrow iMOS were sorted from naïve WT mice and transferred into L. donovani infected STAT1KO mice at 24 h pi, Parasitic loads were analyzed at 20 dpi. (D) Splenocytes were re-stimulated with L. donovani antigen for 72 h, proliferation was avaluated by using Alamar Blue. (E,F) Percentages of Ly6Chi (Ly6Chi Ly6G- CD11b+), Ly6Clow (Ly6Clow Ly6G-CD11b+) and PMN (Ly6C+ Ly6G+ CD11b+) in spleen and liver respectively. (G–I) Supernatants from antigen re-stimulated splenocytes were evaluated for IFNγ, IL-10 and IL-17 production by ELISA. Data represented from 1 of 3 independent experiments. n = 5, *p < 0.05.
