Figure 5

Administration of CCR2 antagonist reduced monocyte recruitment and parasitic loads. L. donovani infected mice were treated with CCR2 antagonist RS-504393, once daily from day 1 to day 30 pi (CCR2A) or once daily from day 20 to day 30 pi (CCR2B) respectively. Flow cytometry analysis of frequencies of Ly6C^hi cells in spleen (Fig. 5A) and liver (Fig. 5E). Flow cytometry analysis of frequencies of Ly6C^low cells in spleen (Fig. 5B) and liver (Fig. 5F). Data shown is from one of the three experiments (n = 4). Spleen weight (Fig. 5C) and liver weight (Fig. 5G) percentages of body weight were represented at 30 dpi. Combined data from two independent experiments N = 8, *p < 0.05 **p = 0.01 ***p = 0.001. (I,J) Representative images and Kinetics of Luciferin-expression L. donovani was analyzed by IVIS imaging system at the indicated time points. Data representative of the mean ± SEM of photons/second/cm² (n = 3).