Figure 6
From: Ly6Chi inflammatory monocytes promote susceptibility to Leishmania donovani infection
CCR2 antagonist reduced percentages of IFNγ+ IL10+ double producer CD4 cells. Splenocytes were harvested from L. donovani infected, CCR2A, CCR2B and vehicle treated mice at 30 dpi, re-stimulated with LdAg for 72 h, and release of cytokines was evaluated in the culture supernatants. (A) IFNγ, (B) IL-4 and (C) IL-10 release was evaluated by ELISA. (D) Representative dot plots showing IFNγ and/or IL10 production by CD4+ gated cells. Analysis of (E) frequencies of IFNγ IL-10 double producers CD4+ cells in the spleen, (F) IFNγ CD4+ producers cells, and (G) total CD4+ cells n = 4. (H) Proliferation analysis by alamar blue of splenocytes stimulated 72 h with L. donovani antigens. n = 5, *p < 0.05 **p = 0.01.
