Figure 7 | Scientific Reports

Figure 7

From: Increased synthesis and deposition of extracellular matrix proteins leads to endoplasmic reticulum stress in the trabecular meshwork

Figure 7

Dex-induced extracellular ECM leads to ER stress in primary human TM cells. (A) Primary human TM cells (n = 2 strains) were grown on chamber slides and treated with either Veh or Dex for 5 days. Upon decellularization, wells were stained with fibronectin and DAPI, to make ensure successful decellularization. An increased deposition of fibronectin is predominant in Dex treated cells compared to Veh treated cells. Absence of DAPI further ensures lack of cells after decellularization process. (B) Primary human TM cells (n = 2 strains) were grown on decellularized ECM for 5 days and stained with KDEL to detect ER stress. Induction of KDEL and fibronectin was observed in TM cells cultured on ECM derived from Dex-treated TM cells. Scale bar = 50 μm.

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