Figure 8 | Scientific Reports

Figure 8

From: Increased synthesis and deposition of extracellular matrix proteins leads to endoplasmic reticulum stress in the trabecular meshwork

Figure 8

TM cells are sensitive to ER stress from Dex-induced abnormal ECM compared to other ocular cells: Primary human TM cells (A), RPE (B) and corneal fibroblasts (C) were treated with Veh or Dex for 3 days and immunostained for fibronectin and KDEL. Although fibronectin was increased in all of these cell types, ER stress was only observed in TM cells. (D) Human GTM3 or ARPE-19 cells were treated with Veh or Dex for 3 days. Fibronectin and chronic ER stress markers ATF4 and CHOP were examined in cellular lysates (n = 2). Although Dex increased fibronectin in both GTM3 and ARPE-19 cells, chronic ER stress was only observed in GTM3 cells indicating that TM cells are more prone to ER stress from Dex-induced ECM accumulation. (E) GTM-3 or ARPE-19 cells were treated with cellular fibronectin (cFn) for 2 days and ER stress was examined by Western blot analysis.

Back to article page